This is the second edition of a very successful textbook that combines an illustrated, narrative text with 12 rigorously tested, reliable lab experiments in molecular biology, developed at the internationally renowned Dolan DNA Learning Center of Cold Spring Harbor Laboratory. Proven through more than 10 years of teaching in research and non-research colleges and universities, junior colleges, community colleges, and advanced high school biology programs, DNA Science has been successfully integrated into introductory biology, general biology, genetics, microbiology, cell biology, molecular genetics and molecular biology courses. The Text has been completely revised, extensively rewritten, and updated. The new coverage extends to the completion of the draft sequence of the human genome and the enormous impact these and other sequence data are having on medicine, research, and industry. The laboratory experiments cover basic techniques of gene isolation and analysis that will be successful even in the hands of teachers and students with no prior experience. Extensive prelab notes at the beginning of each experiment explain how to schedule and prepare, while flowcharts and icons make the protocols easy to follow. The laboratory course is completely supported by quality-assured products from the Carolina Biological Supply Company, from bulk reagents, to usable reagent systems, to single-use kits, thus satisfying a broad range of teaching applications.